Zeta potential is interesting macro parameter of charge distribution on the surface, as Stern electric double layer. Titration curve, as zeta potential dependence on pH value, is determined from electro-kinetic analyzer. A principle is based on the correlation between the electrolyte pressure difference and the potential difference of the sample streaming cell. Based on Fairbrother-Mastin and following Smoluchovski equation, the zeta potential is proportional to dU/dp gradient, viscosity, permitivity, and conductivity.